DNA-PCR test is the polymerase chain reaction test in which the viral core region is amplified and visualized.

Deoxyribonucleic acid or DNA amplification is monitored in real-time as the Polymerase Chain Reaction progresses.

Deoxyribonucleic acid or DNA amplification is monitored in real-time as the Polymerase Chain Reaction progresses.In the case of TaqMan assays, the real-time polymerase chain reaction(RT-PCR), the amplification of Deoxyribonucleic acid(DNA) is visualized in real-time, the PCR reaction continues by using fluorescent dye or a sequence-specific DNA probe labeled with a fluorescent molecule and a quencher molecule. There is an evolution in nucleic acid amplification technology since it was invented and revolutionized the world. But PCR, including nested PCR and real-time PCR, has their limitation of the cycling for heating and cooling with a need for highly precise equipment. PSR is a novel isothermal method that fulfills the requirements of the World Health Organization (WHO) guidelines for the development of diagnostic systems.

Isothermal gene amplification techniques are increasingly becoming popular in resource-limited settings. In recent years various isothermal amplification techniques have been reported for amplification of nucleic acid, including both DNA as well as RNA. A novel isothermal method Polymerase spiral reaction(PSR) uses two primers and a DNA polymerase enzyme for amplification.